diff --git a/algorithms.py b/algorithms.py
index 9846fe3..ce0e904 100644
--- a/algorithms.py
+++ b/algorithms.py
@@ -998,9 +998,13 @@ class AlgorithmManager:
             config_manager = get_config_sync_manager()
             bindings = config_manager.get_bindings_from_redis("")
 
+            # 收集当前配置中有效的 (roi_id, bind_id) 组合
+            valid_keys = set()
+
             for bind in bindings:
                 bind_id = bind.get("bind_id")
                 roi_id = bind.get("roi_id")
+                valid_keys.add((roi_id, bind_id))
 
                 if preserve_state:
                     # 仅更新参数，不重置状态
@@ -1012,6 +1016,32 @@ class AlgorithmManager:
                     if self.load_bind_from_redis(bind_id):
                         count += 1
 
+            # 清理内存中已被删除的算法实例
+            removed_count = 0
+            with self._update_lock:
+                for roi_id in list(self.algorithms.keys()):
+                    for key in list(self.algorithms[roi_id].keys()):
+                        # key 格式: "roi_id_bind_id"
+                        if key.startswith(roi_id + "_"):
+                            bind_id = key[len(roi_id) + 1:]
+                            if (roi_id, bind_id) not in valid_keys:
+                                for algo in self.algorithms[roi_id][key].values():
+                                    algo.reset()
+                                del self.algorithms[roi_id][key]
+                                # 清除注册缓存
+                                self._registered_keys = {
+                                    k for k in self._registered_keys
+                                    if not (k[0] == roi_id and k[1] == bind_id)
+                                }
+                                removed_count += 1
+                                logger.info(f"清理已删除的算法实例: {key}")
+                    # 如果 roi 下已无算法实例，清理空字典
+                    if not self.algorithms[roi_id]:
+                        del self.algorithms[roi_id]
+
+            if removed_count > 0:
+                logger.info(f"已清理 {removed_count} 个孤立算法实例")
+
             logger.info(f"已重新加载 {count} 个算法配置 (preserve_state={preserve_state})")
             return count
         except Exception as e:
diff --git a/config/database.py b/config/database.py
index b1eda4e..d23be56 100644
--- a/config/database.py
+++ b/config/database.py
@@ -899,7 +899,17 @@ class SQLiteManager:
         except Exception as e:
             logger.error(f"删除ROI算法绑定失败: {e}")
             return 0
-    
+
+    def get_all_bind_ids(self) -> List[str]:
+        """获取所有算法绑定的 bind_id 列表（用于清理孤立绑定）"""
+        try:
+            cursor = self._conn.cursor()
+            cursor.execute("SELECT bind_id FROM roi_algo_bind")
+            return [row[0] for row in cursor.fetchall()]
+        except Exception as e:
+            logger.error(f"获取所有绑定ID失败: {e}")
+            return []
+
     def log_config_update(
         self,
         config_type: str,
diff --git a/core/config_sync.py b/core/config_sync.py
index 7bad3e8..75672ae 100644
--- a/core/config_sync.py
+++ b/core/config_sync.py
@@ -640,6 +640,15 @@ class ConfigSyncManager:
                         removed += 1 + bind_count
                         logger.info(f"清理旧 ROI: {old_roi_id} (含 {bind_count} 条算法绑定)")
 
+            # 清理孤立的算法绑定（ROI 仍存在但绑定已被删除的情况）
+            if incoming_bind_ids:
+                existing_bind_ids = self._db_manager.get_all_bind_ids()
+                for old_bind_id in existing_bind_ids:
+                    if old_bind_id not in incoming_bind_ids:
+                        self._db_manager.delete_roi_algo_bind(old_bind_id)
+                        removed += 1
+                        logger.info(f"清理孤立算法绑定: {old_bind_id}")
+
             if removed > 0:
                 logger.info(f"旧数据清理完成: 共删除 {removed} 条过期记录")